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| Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire. |
| Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009 |
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| Ranzani-Paiva,Maria José Tavares; Tabata,Yara Aiko; Eiras,Augusta Cocuzza das. |
| This Study was carried out at the Salmoniculture Experimental Station "Dr. Ascanio de Faria", in Campos do Jordão, São Paulo. Brazil. The aim was to compared the hematology of triploid and diploid rainbow trout, Oncorhynchus mykiss Walbaum, 1792, by sex and gonadal maturation stages. Blood was obtained from 144 individuais for determinati-ons of: haemoglobin rate (Hb), haematocrit (Ht), erythrocyte count (Er), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MHC), mean corpuscular hemoglobin concentration (MCHC), and differential leucocyte counts. The ploid was determinated by measuring the erythrocyte major axis, in fresh and stainncd smears. Triploid rainbow trout had a large eiythrocyte diameter, mean erythrocyte volume and mean haemoglobin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oncorhynchus mykiss; Diploid; Triploid; Hematolology; Rainbow trout. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000400026 |
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